Director:

Denise Faustman, MD, PhD

Contact Person: Doug Burger
Phone: 617-724-9510
Fax: 617-726-4095
Email:
dburger@partners.org

The purpose of this Immunology Flow Cytometry Core of the Boston Area Diabetes Endocrinology Research Center (BADRC) is to provide flow cytometric techniques in a cost effective manner to the diabetes research community. The core is committed to providing to investigators both training and a diversity of state-of-the-art flow cytometric techniques. The Core will assist investigators in applying a variety of tough and cumbersome immunologic techniques and offers in-depth training of new methods. .We provide fundamental services to the Boston-Cambridge diabetes based research community which include

1. Flow cytometer access.
2. Flow cytometry training, education and experiment design assistance.
3. Flow cytometry sample analysis
4. Flow cytometry data interpretation

The flow cytometer previously available for sophisticated sorting services, (i.e., Epics Elite Flow Cytometer) has been supplemented through the purchase, last year of second flow cytometer (BD FASC Calibur Flow Cytometer). Furthermore, the Core has purchased two MASC magnetic field separation machines appropriate for large scale and very rapid cell separations

Services Provided:
All aspects of flow cytometry, including teaching of fellows on applications, methods of sample preparation and project feasibility, instruction and hands-on assistance with data interpretation, instructions of manuscript preparations of flow cytometry data.

  • Single, double and triple color immunofluorescence analysis
  • Single, double and triple color cellular sorting
  • Measurement of intracellular calcium signaling using Indo-1
  • DNA cell cycle analysis
  • Apoptosis tracking by propidium iodide staining


Service Fee: $75/Hour Non-Core Users; $50/Hour Core Users

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